Monoclonal Antibody Production Service
About the service
Monoclonal antibodies (mAbs) are antibodies produced through cell fusion techniques, combining B lymphocytes with Balb/c mouse myeloma cells. These antibodies specifically recognize a single epitope, making them valuable tools for research, diagnosis, and treatment purposes. The process of monoclonal antibody production involves multiple steps. First, Balb/c mice are injected several times with the antigen until the desired immune response is achieved. Then, the mouse is euthanized, and the spleen is then removed from the mouse's body. The cells from the spleen are subsequently extracted for further processing. Spleen cells are fused with Balb/c mouse cancer cells known as SP2/0 using polyethylene glycol (PEG). In this case, the presence of aminopterin in the HAT medium induces Sp2/0 cell death, while the unfused spleen cells lack the ability to proliferate and eventually perish after a few days. Only the hybridoma cells, resulting from the fusion of Sp2/0 cells and spleen cells, possess the capability to grow and survive in the HAT medium. After multiple rounds of cell culture medium replenishment (feeding) to support hybridoma growth, once the hybridoma cells have reached a sufficient growth density, the supernatant is subjected to testing using an indirect ELISA assay to identify the wells that contain hybridomas producing the desired monoclonal antibodies. To obtain a stable hybridoma, positive wells in the ELISA test are subjected to cloning using limiting dilution, ensuring that only one cell is present in each well. The cloning process is repeated four times, ensuring that all wells containing single cells must provide positive ELISA test result after each round of cloning. Since the initial cell clone tested positive and subsequent clones derived from it also produced the same results, the resulting product is referred to as a monoclonal antibody. Both the supernatant from hybridoma cell culture and ascites can be used for monoclonal antibody purification. Thick supernatant can be obtained by culturing hybridoma cells in large flasks or bioreactors, while ascites production involves injecting hybridoma clones into the peritoneal cavity of Balb/c mice.
Our hybridoma core laboratory is equipped to provide services to research centers, investigators, and students throughout the country. Our team consists of experienced experts and academic staff members who are dedicated to meeting your needs.
Hybridoma cell culture services:
- Cultivation of suspension and adherent cells
- Supply of various types of animal cells
- Creation of single cells and injection into mice to induce tumor growth
- Antigen injection into laboratory animals
- Conducting ELISA tests
- Monoclonal antibody production (hybridoma)
- Ascites production
- Antibody purification using protein G columns
- Construction of affinity chromatography columns (Affinity Chromatography)
- Antibody purification using affinity chromatography columns
Laboratory Supervisor:
Dr. Ali Ahmad Bayat (Faculty member of Avicenna Research Institute); Email: bayat @avicenna.ac.ir
Laboratory Assistant:
Location of Laboratory:
Our hybridoma core laboratory is situated on the first floor of Avicenna Research Institute, located within Shahid Beheshti University.
Working Schedule:
The laboratory operates from Saturday to Wednesday, between 8:00 AM and 4:00 PM.
Service Policy and Costs:
The cost of tests or services will vary depending on the sample type and specific testing requirements. Therefore, upon the initial inquiry, the laboratory will provide information regarding the cost of the requested test.
Sample Submission:
To ensure smooth processing, please coordinate with the laboratory assistant at least 48 hours before sending the sample.
