NanoDrop spectrophotometer is renowned for its ability to measure low volumes in the field of spectrophotometry, particularly in biological sciences. Leveraging the innovative "Acclaro smart technology," this device enables quantitative and qualitative measurements of various samples, including RNA, DNA, peptides, and proteins. It can identify sample contamination with just 1-2 microliters of volume in a few seconds and provide results in terms of optical density (OD), concentration, and a comparative chart. Additionally, the device's software allows users to export the results in formats such as Excel files. The NanoDrop spectrophotometer covers a wavelength range of 200-800 nm. The NanoDrop spectrophotometer has applications in genomics, proteomics, drug discovery, molecular detection, and biomanufacturing.

In this laboratory, we utilize the Thermo Scientific NanoDrop 2000. The range of tests that can be conducted with laboratory devices:

• Nucleic acid (Double and single stranded DNA / RNA)
• Microarray analysis
• UV-Vis spectroscopy
• Protein A280 measurement
• Proteins and label analysis
• BCA protein assay
• Lowry protein assay
• Bradford protein assay
• Pierce protein assay
• Cell culture analysis

The measurement of concentration is a crucial step in various experiments involving different samples. Without knowing the concentration of a substance dissolved in a solution, it is not possible to perform accurate tests on that sample. Spectrophotometry is an excellent technique for measuring the concentration of various substances in a solvent. This technique relies on the irradiation of the sample with light of a specific wavelength (in the ultraviolet and visible range) and then measuring the amount of light absorbed by the solution. Thermo Scientific NanoDrop 2000 utilizes a xenon lamp as its light source and a 2048-element linear silicon CCD array as its detector. This spectrophotometer offers a wide range of spectral coverage (190-850 nm) and can measure the concentration and quality of nucleic acids within the range of 2-3700 ng/ml without the need for sample dilution. It provides an optical absorption accuracy of 0.003 Abs.

Thermo Scientific NanoDrop 2000 offers several advantages, including the ability to measure cell density, indirectly measure protein mixture concentration by generating a standard curve, determine the concentration of cy-dye labels for microarray use, and automatically store all measurements separately based on absorbance per nanometer. Furthermore, it features a monitoring and troubleshooting system that allows users to assess the physical quality of the device's components. The spectrophotometer also incorporates error detection through warning signs and ensures result accuracy through internal sensor and digital photo analysis.

The table below presents a comparison of the optimal wavelengths utilized in various methods.